| 质粒名称: | pLenti CMV GFP Puro (658-5) |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 17448 |
| 存储实验室: | Eric Campeau, Paul Kaufman |
|
载体骨架基本信息 |
|
| 载体名称: | p156RRL-sinPPT-CMV-GFP-PRE/Nhe I |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 7889 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | GFP,enhanced GFP |
| 物种来源: | -- |
| 插入大小: | 719 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BamHI(未破坏) |
| 3' 克隆位点: | Sal I(未破坏) |
| 5' 测序引物 | CMVforw |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pLenti CMV GFP Puro (658-5) was a gift from Eric Campeau & Paul Kaufman (Addgene plasmid # 17448 ; http://n2t.net/addgene:17448 ; RRID:Addgene_17448) |
| 参考文献部分: | A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD.. PLoS One. 2009 Aug 6;4(8):e6529. 10.1371/journal.pone.0006529 PubMed 19657394 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS pLenti CMV GFP Puro (658-5) 8619 bp ds-DNA circular SYN 25-NOV-2019
DEFINITION 3rd gen lentiviral eGFP expression vector, CMV promoter, Puro.
ACCESSION .
VERSION .
KEYWORDS pLenti CMV GFP Puro (658-5)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8619)
AUTHORS Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO,
Campisi J, Yaswen P, Cooper PK, Kaufman PD.
TITLE A versatile viral system for expression and depletion of proteins in
mammalian cells.
JOURNAL PLoS One. 2009 Aug 6;4(8):e6529.
PUBMED 19657394
REFERENCE 2 (bases 1 to 8619)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Nov 25, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8619
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 26..130
/gene="bla"
/label=AmpR promoter
CDS 131..991
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(349..368)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 1162..1750
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 1651..1670
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 1904..1921
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 2038..2059
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2074..2104
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2112..2128
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2117..2139
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 2136..2152
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 2136..2152
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 2171..2191
/label=T3
/note="T3 promoter, forward primer"
promoter 2173..2191
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 2219..2445
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 2446..2626
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 2673..2798
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 3291..3524
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 4020..4136
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
enhancer 4159..4462
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 4463..4666
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 4617..4637
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 4663..4687
/label=LNCX
/note="Human CMV promoter, forward primer"
regulatory 4756..4765
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 4762..5481
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(4807..4828)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(5068..5087)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 5415..5436
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 5497..6085
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5550..5570)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5968..5979)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
promoter 6099..6598
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(6134..6157)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 6531..6550
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
CDS 6619..7218
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(6619..6638)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 7115..7135
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
LTR 7356..7589
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 7661..7782
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7698..7717)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7752..7771
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 7822..7957
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 7884..7903
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(7977..7996)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(7978..7996)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(8006..8023)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(8006..8022)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8015..8037)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 8164..8619
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(8251..8270)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 8461..8482
/label=F1ori-F
/note="F1 origin, forward primer"
ORIGIN
1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
1561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
2221 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
2281 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
2341 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
2401 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
2461 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
2521 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
2581 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
2641 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
2701 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
2761 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
2821 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
2881 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
2941 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
3001 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
3061 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
3121 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
3181 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
3241 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
3301 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
3361 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
3421 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
3481 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
3541 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
3601 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
3661 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
3721 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
3781 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
3841 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
3901 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
3961 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact
4021 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
4081 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg
4141 ataagcttgg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
4201 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
4261 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
4321 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
4381 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
4441 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
4501 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
4561 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
4621 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg
4681 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgactcta
4741 gaggatccac cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
4801 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
4861 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
4921 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
4981 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
5041 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
5101 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
5161 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
5221 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
5281 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
5341 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
5401 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
5461 atggacgagc tgtacaagta aagcggccgc gtcgacaatc aacctctgga ttacaaaatt
5521 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
5581 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
5641 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
5701 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
5761 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
5821 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
5881 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
5941 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
6001 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
6061 atctcccttt gggccgcctc cccgcctgga attctaccgg gtaggggagg cgcttttccc
6121 aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg
6181 cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg
6241 tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca
6301 gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat
6361 ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct
6421 ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag
6481 gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac
6541 gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac
6601 ctgcagccca agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
6661 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
6721 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
6781 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
6841 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
6901 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
6961 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
7021 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
7081 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
7141 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
7201 cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca
7261 cgaccccatg catctcgagg gcccggtacc tttaagacca atgacttaca aggcagctgt
7321 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctagctc actcccaacg
7381 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
7441 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
7501 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
7561 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
7621 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
7681 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
7741 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
7801 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
7861 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
7921 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
7981 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
8041 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
8101 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
8161 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
8221 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
8281 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
8341 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
8401 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
8461 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
8521 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
8581 ttaacgcgaa ttttaacaaa atattaacgc ttacaattt
//
